Exploiting Pan Influenza A and Pan Influenza B Pseudotype Libraries for Efficient Vaccine Antigen Selection

We developed an influenza hemagglutinin (HA) pseudotype library encompassing Influenza A subtypes HA1-18 and B (both lineages) to be employed in microneutralization (pMN) assays. The pMN is highly sensitive specific for detecting virus-specific neutralizing antibodies against viruses can used assess antibody functionality vitro. Here we show the production of these viral HA pseudotypes their employment as substitutes wildtype neutralization demonstrate utility serum responses vaccination with ability evaluate cross-subtype elicited by vaccinating antigens. Our findings may inform further preclinical studies involving immunization dosing regimens mice help creation selection better antigens vaccine design. These harnessed meet strategic objectives that contribute strengthening global surveillance, expansion seasonal prevention control policies, pandemic preparedness response.